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Bloodstain Pattern Analysis

Page history last edited by barbara gibson 5 years, 8 months ago



Bloodstain Pattern Analysis

"It will have blood, they say; blood will have blood" -William Shakespeare, Macbeth




  • Section 1a. Vocabulary


 platelets    spatter   back spatter    area of convergence exit wound      void   drip trail pattern
   bloodstain   satellite spatter     low-velocity spatter   area of origin    cast off   transfer pattern   grid method
  droplet    angle of impact     medium-velocity spatter     string method  arterial spray    flow pattern    perimeter rule method
  forward spatter   impact spatter   high-velocity spatter   entrance wound    expirated blood pattern    skeletonization  erythrocytes
 leukocytes hemoglobin  luminol  precipitin           



  • Section 1b. Mastery Objectives and Critical Skills
  • Identify blood at the crime scene
  • Process blood samples from the crime scene (type, DNA, etc)
  • Categorize the blood spatter patterns as satellite spatter, impact spatter, forward spatter, back spatter, low-velocity spatter, medium-velocity spatter, high-velocity spatter, expirated blood pattern, transfer pattern, flow pattern, drip trail pattern and skeletonization 
  • Identify the 12 categories of patterns
  • Describe the 3 methods for identifying blood at a crime scene
  • Discuss the 3 types of information one gets from blood at a crime scene 




  • Section 4 – Outside Class Assignments: 
  1. Concept Maps 
      1.  droplet, platelets, leukocytes, erythrocytes, spatter, satellite spatter, angle of impact, bloodstain
      2. bloodstain pattern, impact spatter, forward spatter, back spatter, low velocity spatter, medium-velocity spatter, high-velocity spatter, low-velocity spatter
      3. area of convergence, area or origin, string method, entrance wound, exit wound, cast-off, arterial spray
      4. expirated blood pattern, void, flow patterns, skeletonization, transfer pattern, drip trail pattern, grid method, perimeter rule method


     2.  What characteristics of the criminal can be identified from the blood found at the scene? Age, gender and DNA.


     3a. What are the three types of analyses used to process blood in a lab? Conventional Serological Analysis, Restriction Fragment Length      Polymorphism (RFLP), and Polymerase Chain Reaction (PCR). 


     3b. How are they defined? Conventional Serological: Analysis of the proteins, enzymes, and antigens present in the blood.

     RFLP: Direct analysis of certain DNA sequences present in the white blood cells. 

     PCR: Analysis of certain DNA sequences that have been copied multiple times to a detectable level 


     4. Match the terms of the blood spatter with the image that corresponds with it. 

Name of Pattern   Picture (a, b, f, i, l) copyright FORident Software: http://hemospat.com/bloodstain-pattern-analysis-terminology
1. satellite spatter  a. 
2. high-velocity spatter  b. 
3. back spatter  c.  
4. skeletonization  d. 
5. flow pattern  e.  
6. impact spatter  f.  
7. drip trail pattern  g. 
8. low-velocity spatter  h. 
9. void pattern  i.
10. transfer pattern  j. 
11. forward spatter  k. 
12. expirated blood pattern  l. 
13. medium-velocity spatter  m. 

(Answers: 1f, 2e, 3h, 4g, 5i, 6c, 7k, 8d, 9l, 10b, 11m, 12a, 13j) 


     5. What are the 3 methods for identifying blood at the crime scene? Luminol Tests, Hydrogen Peroxide and Hydroxide.


     6. What are the three types of information one might get from a crime scene? Height, location and handedness. 


  • Section 5 – Outside Class Activities and Bonus PointsYou may want to include some “stretch” activities that a student who is really excited about the topic can pursue.  Have them visit a place, build a collections, research a related topic.



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